Standard Nuclera Additives
General Information
Uniquely, we have designed our cell-free blend to be tailorable and customizable through the addition of up to two additives that can be used to further optimize expression of your proteins in our system. See a list of our standard additives below:
- Chaperones
- Disulfide bond formation additives
- Cofactors (e.g., FAD/NAD/SAM)
- Metal Ions (e.g., Zn2+, Mg2+, Mn2+, Ca2+)
- 3C protease
The image below can guide you through which additive should be selected for your specific protein:
Standard Nuclera Additives
Uniquely, we have designed our cell-free blend to be tailorable and customizable through the addition of up to two additives that can be used to further optimize expression of your proteins in our system. See a list of our standard additives below:
| Additive | Additive Description | Additive Characteristics |
|---|---|---|
| Additive buffer | HEPES buffer pH 7.5 and surfactant | CFPS reaction buffer, dilution normalization |
| PDI + GSSG Mix | Protein disulfide isomerase and oxidized glutathione | Chaperone and redox modification to oxidizing environment to support disulfide bond formation |
| TrxB1 | Thioredoxin reductase | Protects proteins from oxidative aggregation and inactivation and acts as a reductase in redox regulation |
| DnaK Mix | Chaperone | DnaK mix Chaperone mix to support folding and prevent aggregation |
| Zinc chloride | Zinc chloride solution | Cofactor that can be required for folding, stability, or activity |
| Calcium chloride | Calcium chloride solution | Cofactor that can be required for compaction, folding, stabilization, or activity |
| Manganese chloride | Manganese chloride solution | Cofactor for metalloenzymes for structure and activity |
| Cofactor Mix | Mix of NAD, acetyl CoA, FAD, SAM, and PLP | Cofactors that assist in folding, stability and activity |
| GSSG | Oxidized glutathione | Redox modification to oxidizing environment |
| 3C protease | 3C protease solution | Protease to cleave off the N-terminal solubility tag at the specific aminoacid sequence (LEVLFQ/GP) |
If you need help to desing and choose additives for your experiment, do not hesitate to contact us at techsupport@nuclera.com
Nuclera Technical Support:
UK Phone +44 1223 942 761
US Phone: +1 508-306-1297
Email: techsupport@nuclera.com
Offices:
Nuclera UK (HQ):
One Vision Park, Station Road, Cambridge, CB24 9NP, UK
Nuclera USA:
1000 Technology Park Drive, Suite B, Billerica MA 01821, USA
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